ACANTHAMOEBA SP.S-11 PHAGOCYTOTIC ACTIVITY ON MYCOBACTERIUM LEPRAE IN DIFFERENT NUTRIENT CONDITIONS

Authors

  • Sepling Paling Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;
  • Ratna Wahyuni Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;
  • DEA Ni’matuzahroh Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;
  • Dwi Winarni Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;
  • M. KES Iswahyudi Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;
  • Linda Astari Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Surabaya 60286, Indonesia.
  • Dinar Adriaty Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;
  • Indropo Agusni Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Surabaya 60286, Indonesia.
  • Shinzo Izumi Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

DOI:

https://doi.org/10.21010/Ajid.v12i1S.5

Keywords:

Acanthamoeba sp., Mycbacterium leprae, Nutrient, Phagocytosis, Real Time PCR

Abstract

Background: Mycobacterium leprae (M. leprae) is a pathogenic bacterium that causes leprosy. The presence of M. leprae in the environment is supported by microorganisms that act as the new host for M. leprae. Acanthamoeba’s potential to be a host of M. leprae in the environment. Acanthamoeba sp. is Free Living Amoeba (FLA) that classified as holozoic, saprophytic, and saprozoic. The existence of nutrients in the environment influence Acanthamoeba ability to phagocytosis or pinocytosis. This study is aimed to determine Acanthamoeba sp.S-11 phagocytic activity to Mycobacterium leprae (M. leprae) which cultured in non-nutrient media and riched-nutrient media. Materials and Methods: This research conducted by culturing Acanthamoeba sp.S-11 and M. leprae on different nutrient media conditions. M. leprae intracellular DNA were isolated and amplified by M. leprae specific primers through Real Time PCR (Q-PCR). Result: The results showed that Acanthamoeba co-cultured on non-nutrient media were more active to phagocyte M. leprae than on rich-nutrient media. Conclusion: The use of non-nutrient media is recommended to optimize Acanthamoeba sp. phagocytic activity to M. leprae. Keywords: Acanthamoeba sp., Mycbacterium leprae, Nutrient, Phagocytosis, Real Time PCR Introduction Mycobacterium leprae (M. leprae) is a pathogenic

Author Biographies

Sepling Paling, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;

Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;

Ratna Wahyuni, Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

DEA Ni’matuzahroh, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;

Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;

Dwi Winarni, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;

Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia;

M. KES Iswahyudi, Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

Linda Astari, Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Surabaya 60286, Indonesia.

Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Surabaya 60286, Indonesia.

Dinar Adriaty, Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

Indropo Agusni, Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Surabaya 60286, Indonesia.

Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga, Surabaya 60286, Indonesia.

Shinzo Izumi, Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia;

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Published

2018-03-07

How to Cite

Paling, S., Wahyuni, R., Ni’matuzahroh, D., Winarni, D., Iswahyudi, M. K., Astari, L., Adriaty, D., Agusni, I., & Izumi, S. (2018). ACANTHAMOEBA SP.S-11 PHAGOCYTOTIC ACTIVITY ON MYCOBACTERIUM LEPRAE IN DIFFERENT NUTRIENT CONDITIONS. African Journal of Infectious Diseases (AJID), 12(1S), 44–48. https://doi.org/10.21010/Ajid.v12i1S.5

Issue

Vol. 12 No. 1S (2018): Special Issue

Section

Articles

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