@article{Manela_Susanti_Tjong_Yudianto_2022, title={GENETIC ANALYSIS 21 SHORT TANDEM REPEATS (STR) LOCUS IN MINANGKABAU POPULATION, WEST SUMATERA, INDONESIA}, volume={16}, url={https://athmsi.org/journals/index.php/AJID/article/view/5801}, DOI={10.21010/Ajid v16i2.4}, abstractNote={<p><strong>Background: </strong>Minangkabau is the majority ethnic group in West Sumatra, Indonesia. West Sumatra is a disaster area, especially earthquakes and the potential for a tsunami. Allele frequency for 21 short tandem repeat locus and genetic variation are not well known. This data is essential for calculating the Paternity Index and Match Probability for forensic identification.</p> <p><strong>Materials and methods: </strong>This was an observational study. We analyze the GlobalFiller STR loci in 25 unrelated individuals from Minangkabau ethnic group. The DNA was extracted using a Prefiller kit and amplified with a Global Filler kit by a GeneAmp PCR System, followed by capillary electrophoresis using ABI Prism 3500 Genetic Analyzer. Data analysis was performed by using Easy DNA and FORSTAT software.</p> <p><strong>Results: </strong>We observed 162 alleles with allele frequencies between 0.02 – 0.36. The highest expected heterozygosity and the highest power of discrimination were at the SE33 loci, and the highest match probability was at the D2S441 locus. The Chi-square test showed that all STR loci followed Hardy–Weinberg equilibrium (p > 0.05). All loci were highly polymorphic (PIC > 0.5). The combined discrimination capacity of each locus in the population was 99,999%.</p> <p><strong>Conclusion: </strong>The 21 STR loci are useful for forensic analysis and population genetic studies of the Minangkabau population.</p> <p><strong> </strong></p>}, number={2}, journal={African Journal of Infectious Diseases (AJID)}, author={Manela, Citra and Susanti, Rika and Tjong, Djong Hon and Yudianto, Ahmad}, year={2022}, month={May}, pages={35–41} }