TY - JOUR AU - Tasiam, Ezrani AU - Primaharinastiti, Riesta AU - Ekasari, Wiwied PY - 2020/07/31 Y2 - 2024/03/29 TI - IN VITRO ANTIMALARIAL ACTIVITY AND TOXICITY STUDIES OF JOHAR (CASSIA SIAMEA) LEAVES FROM THREE DIFFERENT LOCATIONS JF - African Journal of Infectious Diseases (AJID) JA - Afr. J. Infect. Dis. VL - 14 IS - 2 SE - Articles DO - 10.21010/Ajid.v14i2.4 UR - https://athmsi.org/journals/index.php/AJID/article/view/5539 SP - 23-29 AB - <p><strong>Background:</strong>&nbsp;Antimalarial activity of <em>C</em><em>assia</em><em>&nbsp;</em><em>siamea</em>&nbsp;leaves has been proven by the active compound that has been found, i.e.&nbsp;Cassiarin A. It is known that the quantity of the content of a compound that has the potential&nbsp;as a raw material for medicine can be influenced by various factors including differences in plant origin. This study aims at comparing the antimalarial activity and toxicity of <em>C.siamea</em>&nbsp;leaves from three regions with different meters location values above sea level (asl), i.e Pariaman (1,000 m asl), Palu (60 m asl), and Surabaya (2 m asl).</p><p><strong>Materials and Methods</strong>: The materials used in this study were Johar leaves from Pariaman, Surabaya, and Palu extracted with n-hexane, and 90% ethanol containing 1% tartaric acid. The antimalarial activity test was done with <em>Plasmodium falciparum</em>&nbsp;3D7. The toxicity test applied <em>MTT ELISA</em>&nbsp;method.</p><p><strong>Results:</strong>&nbsp;<em>C.siamea </em>leaf that had highest&nbsp;antimalarial activity came from Pariaman with IC<sub>50</sub>&nbsp;value of 0.006μg/ml, then from Palu was 0.037μg/ml, and the lowest antimalarial activity was from Surabaya that was 0.09μg/ml . In testing the toxicity to get CC<sub>50</sub>, the highest toxicity came from Surabaya with CC<sub>50 </sub>value of 135.81μg/ml, Pariaman with CC<sub>50 </sub>value of 220.82&nbsp;μg/ml, and the least toxic came from Palu with CC<sub>50 </sub>value of 235.52μg/ml&nbsp;.&nbsp;</p><p><strong>Conclusion</strong>: <em>C.siamea</em>&nbsp;leaf obtained from Pariaman had a selectivity index value that satisfies&nbsp;the requirements of a promising antimalarial effect.</p> ER -