CHEMOPREVENTIVE EFFECT OF COUSINIA SHULABADENSIS ATTAR & GHAHRAMAN ETHANOL EXTRACT

Authors

  • Ahmad R. Shahverdi
  • Mohammad R. Khoramizadeh
  • Mohammad H. Ghahramani
  • Ardeshir Golyaee
  • Farideh Attar
  • Ahmad Ghahraman

Keywords:

anti-invasive activity, chemoprevention, cytotoxicity, Cousinia shulabadensis, matrix metalloproteinases

Abstract

Matrix metalloprotainases (MMPs) play an important role in several pathologic processes such as malignancy in which they facilitate invasion and metastasis and can be targets for anticancer therapies. Here, in this study, we investigated the cytotoxicity effect of Cousinia shulabadensis Attar & Ghahraman extract as well as its impact on MMPs activity using a model of cell line (Fibrosarcoma-Wehi164). To assess anti-invasiveness potentials, a modified zymoanalysis method was used to measure MMP-2 and MMP-9 activities in the conditioned-media. The concentration necessary to produce 50% cell death was >80µg/ml for ethanol extract of Cousinia shulabadensis, while a 23 µg/ml concentration of the diclofenac sodium produced the same effect. The invasion of WEHI 164 cells was considerably inhibited at concentrations > 20 µg/ml by total plant extract. The total extract of the plant did not show high toxicity at all tested concentrations, but demonstrated significant inhibition of MMP activity in dose-response fashion.

Author Biographies

Ahmad R. Shahverdi

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Mohammad R. Khoramizadeh

Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, P.O.Box 14155/6451,Tehran, Iran

Mohammad H. Ghahramani

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran, University of Medical Sciences, Tehran, Iran

Ardeshir Golyaee

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran, University of Medical Sciences, Tehran, Iran

Farideh Attar

Central Herbarium Museums, Faculty of Sciences, Tehran University, Tehran, Iran

Ahmad Ghahraman

Central Herbarium Museums, Faculty of Sciences, Tehran University, Tehran, Iran

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Published

2006-08-28

How to Cite

Shahverdi, A. R., Khoramizadeh, M. R., Ghahramani, M. H., Golyaee, A., Attar, F., & Ghahraman, A. (2006). CHEMOPREVENTIVE EFFECT OF COUSINIA SHULABADENSIS ATTAR & GHAHRAMAN ETHANOL EXTRACT. African Journal of Traditional, Complementary and Alternative Medicines, 4(1), 12–16. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/155

Issue

Vol. 4 No. 1 (2007)

Section

Research Papers

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