ANTIOXIDATIVE POTENTIAL OF DURANTA REPENS (LINN.) FRUITS AGAINST H2O2 INDUCED CELL DEATH IN VITRO

Authors

  • Md. Asaduzzaman Khan Department of Biochemistry, School of Biological Science and Technology, Central South University, Changsha, Hunan, China
  • Mohammad Mijanur Rahman Department of Biochemistry and Molecular Biology, Jahangirnagar University
  • Mousumi Tania Department of Biochemistry, School of Biological Science and Technology, Central South University
  • Nusrat Fatima Shoshee Department of Biochemistry and Molecular Biology, Jahangirnagar University
  • Ai-hua Xu Department of Biochemistry, School of Biological Science and Technology, Central South University
  • Han-chun Chen Department of Biochemistry, School of Biological Science and Technology, Central South University

Keywords:

Duranta repens (Linn.), Oxidative cell death, Lipid per-oxidation, Antioxidant, Phytochemicals

Abstract

The effects of Duranta repens fruits were investigated on H2O2 induced oxidative cell death to evaluate its antioxidative potential in vitro. HEK293T cells were treated with different concentrations [0-1000 µg/ ml] of ethanol extract (E-Ex) and methanol extract (M-Ex) of D. repens for 24h, and then treated with 100 µM H2O2 for 24h. Cell viability, antioxidant parameters of cells, and antioxidant constituents of the extracts were determined. Treatment with limited dose of E-Ex or M-Ex increased the survival rate of H2O2–treated HEK293T cells, however the extra-high dose showed growth inhibitory effect. Treatment with E-Ex or M-Ex protected cellular lipid per-oxidation. In vitro analyses showed the 2,2-diphenyl-1-picrylhydrazyl and H2O2 scavenging activities as well as reducing potential of the extracts. We report here that the limited dose of E-Ex and M-Ex possess antioxidative potential, which can protect H2O2-induced oxidative cell damage.

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Published

2013-03-15

How to Cite

Khan, M. A., Rahman, M. M., Tania, M., Shoshee, N. F., Xu, A.- hua, & Chen, H.- chun. (2013). ANTIOXIDATIVE POTENTIAL OF DURANTA REPENS (LINN.) FRUITS AGAINST H2O2 INDUCED CELL DEATH IN VITRO. African Journal of Traditional, Complementary and Alternative Medicines, 10(3), 436–441. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/1636

Issue

Vol. 10 No. 3 (2013)

Section

Research Papers

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