ANTICANCER CONSTITUENTS AND CYTOTOXIC ACTIVITY OF METHANOL-WATER EXTRACT OF POLYGONUM BISTORTA L.

Authors

  • Azeem Intisar
  • Lingyi Zhang
  • Huiyang Luo
  • Boima Kiazolu
  • Runsheng Zhang
  • Weibing Zhang

Keywords:

cytotoxicity, Polygonum bistorta, phenolic compounds, fatty acids, anticancer activity.

Abstract

This study was specifically designed to identify anticancer constituents in methanol-water extract of Polygonum bistorta L. and evaluate its cytotoxicity. For this purpose methanol-water (40:60 v/v) extract was subjected to conventional preparative high pressure liquid chromatography and 13 fractions were obtained. Constituents of obtained fractions were separated and identified with the help of GC-MS and LC-DAD-ESI-MS. Anticancer phenolic compounds such as gallic acid, protocatechuic acid, p-hydroxybenzoic acid, chlorogenic acid, vanillic acid, syringic acid, catechol, 4-methyl catechol, syringol and pyrogallol and fatty acids such as linoleic acid, myristic acid and palmitic acid were separated from different fractions. Fractions were evaluated for their cytotoxic activity on a rarely studied human hepatocellular carcinoma cell line (HCCLM3). 11 fractions showed good to strong cytotoxicity in a range of 200 µg/mL-800 µg/mL, whereas 2 fractions did not show any activity even at 800 µg/mL and no anticancer constituent was detected from them. 50 percent growth inhibition (GI50) values for five most active fractions were calculated and results were in a range of 86.5 (±3) µg/mL-126.8 (±3) µg/mL. 3 out of these 5 most active fractions were found to contain phenolic content in them whereas all other fractions containing phenolic content did possess cytotoxic activity that may suggest the importance of phenolic constituents in anticancer activity. Moreover, the results also showed a definite dose dependent relationship between amount of fractions and cytotoxic activity.

Author Biographies

Azeem Intisar

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of science and Technology, Shanghai 200237, P. R. China

Weibing Zhang

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of science and Technology, Shanghai 200237, P. R. China

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Published

2012-09-22

How to Cite

Intisar, A., Zhang, L., Luo, H., Kiazolu, B., Zhang, R., & Zhang, W. (2012). ANTICANCER CONSTITUENTS AND CYTOTOXIC ACTIVITY OF METHANOL-WATER EXTRACT OF POLYGONUM BISTORTA L. African Journal of Traditional, Complementary and Alternative Medicines, 10(1), 53–59. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/1659

Issue

Vol. 10 No. 1 (2013)

Section

Research Papers

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