IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITY OF LEAVE EXTRACT OF RAPHANUS SATIVUS IN RATS USING CCL4 MODEL

Authors

  • Shariq Naeem Syed Department of Pharmacology, Maulana Azad Medical College, New Delhi, India 110002
  • Waseem Rizvi Department of Pharmacology, JNMC, AMU, Aligarh, India 202002
  • Anil Kumar Department of Pharmacology, JNMC, AMU, Aligarh, India 202002
  • Khan Ahmad Aijaz Department of Anatomy, JNMC, AMU, Aligarh, India 202002
  • Moin Shagufta Department of Biochemistry, JNMC, AMU, Aligarh, India 202002
  • Ahsan Akif Department of Biochemistry, JNMC, AMU, Aligarh, India 202002

Keywords:

Raphanus sativus, hepatoprotection, CCl4, antioxidant

Abstract

Background: Raphanus sativus is reported to have a variety of biological activities. This work screened the hepato-protective and antioxidant activity of ethanol (ERS), and aqueous (ARS), extracts of leaves of Raphanus sativus in Carbon tetrachloride (CCl4), model in rats. Material and Methods: The extracts were subjected to antioxidant tests (Total reducing power and Total phenolic content), and preliminary phytochemical screening. A pilot study was done on 100 and 300 mg/kg extracts, form which 300 mg was chosen for further experiments. The albino rats (200-250 grams), were divided into 5 groups of 6 animals each (n=6). There were three control groups comprising of normal control (normal saline -1ml/kg), negative control group (CCl4 1ml/kg in olive oil in a ratio of 1:1 v/v), and positive control group (Silymarin 50mg/kg). The Test drugs were given in a dose of 300 mg/kg for both ERS and ARS extract for 7 days. Biochemical parameters (AST, ALT, Alkaline phosphatase, Total Bilirubin), histo-pathological examination of liver and in vivo antioxidant tests [CAT, GSH and MDA] were done. Results: The phytochemical study showed the presence of flavanoids, terpenoids, alkaloids, saponins and sterols. A dose dependent increase in the oxidative potential was observed in both the extracts with total phenolic content 70.1 and 44.4 GAE/g extract for ERS and ARS respectively. ERS 300mg/kg showed a significant (p<0.001) increase in levels of AST, ALT and alkaline phosphatase as compared to negative control (percentage hepatoprotection =45.3%) while ARS 300 mg/kg (p<.01) group showed 30% hepatoprotection. The GSH (p<0.001) and CAT (p<0.05) in ERS and ARS were significantly increased while MDA levels were decreased (P< 0.01), as compared negative control. The findings were confirmed histo-pathological examination. Conclusion: The ethanol and aqueous extract of Raphanus sativus have partial hepatoprotection against CCl4 toxicity.

Author Biographies

Shariq Naeem Syed, Department of Pharmacology, Maulana Azad Medical College, New Delhi, India 110002

Senior Resident Doctor

Waseem Rizvi, Department of Pharmacology, JNMC, AMU, Aligarh, India 202002

Assistant Professor

Anil Kumar, Department of Pharmacology, JNMC, AMU, Aligarh, India 202002

Professor

Khan Ahmad Aijaz, Department of Anatomy, JNMC, AMU, Aligarh, India 202002

Professor

Moin Shagufta, Department of Biochemistry, JNMC, AMU, Aligarh, India 202002

Assistant Professor

Ahsan Akif, Department of Biochemistry, JNMC, AMU, Aligarh, India 202002

junior resident

Downloads

Published

2014-03-29

How to Cite

Syed, S. N., Rizvi, W., Kumar, A., Aijaz, K. A., Shagufta, M., & Akif, A. (2014). IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITY OF LEAVE EXTRACT OF RAPHANUS SATIVUS IN RATS USING CCL4 MODEL. African Journal of Traditional, Complementary and Alternative Medicines, 11(3), 102–106. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/1814

Issue

Section

Research Papers