IMPROVED GROWTH AND COLCHICINE CONCENTRATION IN GLORIOSA SUPERBA ON MYCORRHIZAL INOCULATION SUPPLEMENTED WITH PHOSPHORUS-FERTILIZER

Authors

  • Devendra Kumar Pandey Department of Biotechnology, Lovely Professional University, Phagwara-1144402, Punjab, India
  • Rekha Pandey Dubey Department of Biochemistry, Banaras Hindu University, Varanasi-221005
  • Tabarak Malik Department of Biochemistry, Lovely Professional University-144402, India
  • Joginder Singh Department of Biotechnology, Lovely Professional University, Phagwara-1144402, Punjab, India
  • Abhijit Dey Department of Botany, Presidency University, Kolkata West Bengal

Keywords:

Gloriosa superba, colchicine, Arbuscular mycorrhiza, Phosphorus fertilizer

Abstract

Background: Gloriosa superba produces an array of alkaloids including colchicine, a compound of interest in the treatment of various diseases. The tuber of Gloriosa superba is a rich source of colchicine which has shown anti-gout, anti-inflammatory, and anti-tumor activity. However, this promising compound remains expensive and Gloriosa superba is such a good source in global scale. Increase in yield of naturally occurring colchicine is an important area of investigation. Materials and Methods: The effects of inoculation by four arbuscular mycorrhizal (AM), fungi, Glomus mossae, Glomus fasciculatum, Gigaspora margarita and Gigaspora gilmorei either alone or supplemented with P-fertilizer, on colchicine concentration in Gloriosa superba were studied. The concentration of colchicine was determined by high-performance thin layer chromatography. Results: The four fungi significantly increased concentration of colchicine in the herb. Although there was significant increase in concentration of colchicine in non-mycorrhizal P-fertilized plants as compared to control, the extent of the increase was less compared to mycorrhizal plants grown with or without P-fertilization. This suggests that the increase in colchicine concentration may not be entirely attributed to enhanced P-nutrition and improved growth. Among the four AM fungi Glomus mossae was found to be best. The total colchicine content of plant (mg / plant) was significantly high in plants inoculated with Glomus mossae and 25 mg kg-1phosphorus fertilizer (348.9 mg /plant) while the control contain least colchicine (177.87 mg / plant).

Author Biographies

Devendra Kumar Pandey, Department of Biotechnology, Lovely Professional University, Phagwara-1144402, Punjab, India

Assistant Professor, Department of Biotechnology, Lovely Professional University, Phagwara-1144402, Punjab, India

Rekha Pandey Dubey, Department of Biochemistry, Banaras Hindu University, Varanasi-221005

Department of Biochemistry, Banaras Hindu University, Varanasi-221005

Tabarak Malik, Department of Biochemistry, Lovely Professional University-144402, India

1Department of Biochemistry, Lovely Professional University-144402, India

Joginder Singh, Department of Biotechnology, Lovely Professional University, Phagwara-1144402, Punjab, India

Department of Biotechnology, Lovely Professional University, Phagwara-1144402, Punjab, India

Abhijit Dey, Department of Botany, Presidency University, Kolkata West Bengal

Department of Botany, Presidency University, Kolkota West Bengal

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Published

2014-01-23

How to Cite

Pandey, D. K., Dubey, R. P., Malik, T., Singh, J., & Dey, A. (2014). IMPROVED GROWTH AND COLCHICINE CONCENTRATION IN GLORIOSA SUPERBA ON MYCORRHIZAL INOCULATION SUPPLEMENTED WITH PHOSPHORUS-FERTILIZER. African Journal of Traditional, Complementary and Alternative Medicines, 11(2), 439–446. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/2057

Issue

Section

Research Papers