STUDY ON OPTIMISATION OF EXTRACTION PROCESS OF TANSHINONE ⅡA AND ITS MECHANISM OF INDUCTION OF GASTRIC CANCER SGC7901 CELL APOPTOSIS

Authors

  • Jianhong Hou
  • Jiantai He
  • Xin Jin
  • Tian Hu
  • Yangde Zhang

Keywords:

Tanshinone ⅡA, MTT assay, gastric cancer SGC7901 cells

Abstract

The objective of this paper was to investigate the extraction process of tanshinone ⅡA and its mechanism of induction of gastric cancer SGC7901 cell apoptosis. Extraction process of tanshinone ⅡA was optimised by orthogonal experimental method, and its effect on gastric cancer SGC7901 cell apoptosis was observed using MTT assay and electron microscopy. The optimum extraction process of tanshinone ⅡA was as follows: addition of a 10-fold amount of 80% ethanol, one-time extraction, and extraction time of 45 minutes. The study concluded that tanshinone ⅡA can induce apoptosis of gastric cancer SGC7901 cells.

Author Biography

Jianhong Hou

Department of Hepatic Surgery, National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, Central South University 410008, Hunan Provence, China

Downloads

Published

2013-09-20

How to Cite

Hou, J., He, J., Jin, X., Hu, T., & Zhang, Y. (2013). STUDY ON OPTIMISATION OF EXTRACTION PROCESS OF TANSHINONE ⅡA AND ITS MECHANISM OF INDUCTION OF GASTRIC CANCER SGC7901 CELL APOPTOSIS. African Journal of Traditional, Complementary and Alternative Medicines, 10(6), 456–458. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/2099

Issue

Vol. 10 No. 6 (2013)

Section

Short communications

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.