CHEMICAL COMPOSITION OF ESSENTIAL OILS OF EUGENIA CARYOPHYLLA AND MENTHA SP CF PIPERITA AND THEIR IN VITRO ANTIFUNGAL ACTIVITIES ON SIX HUMAN PATHOGENIC FUNGI

Authors

  • Maximilienne Ascension Nyegue University of Yaoundé I
  • Florentine Marie-Chantal Ndoyé-Foe University of Yaoundé I
  • Sara Essama Riwom University of Yaoundé I
  • Christelle Tembiwe Hockmeni Université of Yaoundé I
  • Francois-Xavier Etoa University of Yaoundé I
  • Chantal Menut University of Montpellier 2

DOI:

https://doi.org/10.21010/ajtcam.v11i6.3

Keywords:

Essential oil, Eugenia caryophylla, Mentha sp cf piperita, antifungal activity, Human pathogenic strains, fungistatic and fungicide.

Abstract

Background: Many fungal infections are responsible for human skin damages, to control their negative action, some aromatic and medicinal plants are traditionally used by local population in Cameroon. The present study was carried out to determine the chemical composition of essential oils of Eugenia caryophylla and Mentha sp cf piperita and their antifungal activity on some human pathogenic fungi. Materials and methods: Essential oils from Eugenia caryophylla and Mentha sp cf piperita were extracted by steam distillation using Clevenger apparatus and the antifungal activity was evaluated on six human pathogenic fungal strains; two yeasts (Candida albicans 1 and Candida albicans 2) and four dermatophytes (Tricophyton rubrum 1, T. rubrum 2, T. violaceum, and T. soudanensis) using modified broth microdilution method M27-A3 and M38-A respectively. Results: The essential oils obtained yielded of 5.9 for Eugenia caryophylla and 0.2% Mentha sp cf piperita respectively. The chemical composition was assigned by GC and GC/SM and showed that E. caryophylla was mainly composed of eugenol (80.0 %), -caryophyllene (8.3%), and eugenol acetate (6.7%) while Mentha sp cf piperita was characterized by piperitone (67.5 %), menthol (10.0 %) and ß-phellandrene (5.8%). The result showed that essential oil of E. caryophylla exhibit the highest antifungal activity with MICs and MFC of 0.25µL/mL and 0.125µL/mL for filamentous fungi and MIC of 0.5 µL/mL for both yeast strains while MFC value was 1 µL/mL for one yeast strain and not determined for the second. MFCs Mentha sp cf piperita essential oil showed a weak activity with a MIC of 2.5 µL/mL on Tricophyton strains while no activity was exhibited on Candida albicans strains. Conclusion: The results of this work can be used to confirm their traditional uses and can also be proposed as natural ingredients to some industries to treat superficial infections.

Author Biographies

Maximilienne Ascension Nyegue, University of Yaoundé I

Department of Microbiology, Dr

Florentine Marie-Chantal Ndoyé-Foe, University of Yaoundé I

Department of Biochemistry

Sara Essama Riwom, University of Yaoundé I

Departement of Microbiology, Dr

Christelle Tembiwe Hockmeni, Université of Yaoundé I

Departement of Biochemistry, Ph.D studient

Francois-Xavier Etoa, University of Yaoundé I

Department of microbiology, Pr

Chantal Menut, University of Montpellier 2

Equipe "Glyco et nanovecteurs pour le ciblage thérapeutique" Institut des Biomolécules Max Mousseron, Faculté de Pharmacie• 15 avenue Charles Flahault, Bâtiment E 2ème étage, BP 14491 34093 Montpellier, France

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Published

2014-10-14

How to Cite

Nyegue, M. A., Ndoyé-Foe, F. M.-C., Riwom, S. E., Hockmeni, C. T., Etoa, F.-X., & Menut, C. (2014). CHEMICAL COMPOSITION OF ESSENTIAL OILS OF EUGENIA CARYOPHYLLA AND MENTHA SP CF PIPERITA AND THEIR IN VITRO ANTIFUNGAL ACTIVITIES ON SIX HUMAN PATHOGENIC FUNGI. African Journal of Traditional, Complementary and Alternative Medicines, 11(6), 40–46. https://doi.org/10.21010/ajtcam.v11i6.3

Issue

Vol. 11 No. 6 (2014)

Section

Research Papers

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