A NEW BIOACTIVE THIOPHENOLIC GLYCOSIDE FROM THE LEAF OF MASSULARIA ACUMINATA (G. DON BULLOCK) EX HOYLE (RUBIACEAE)

Authors

  • Ayodeji Oluwabunmi Oriola Obafemi Awolowo University, Ile-Ife, Nigeria
  • Joseph Adetunji Aladesanmi Obafemi Awolowo University, Ile-Ife, Nigeria
  • T O Idowu Obafemi Awolowo University, Ile-Ife, Nigeria
  • E O Akinkunmi Obafemi Awolowo University, Ile-Ife, Nigeria
  • Efere Martins Obuotor Obafemi Awolowo University, Ile-Ife, Nigeria
  • Makanjuola Ojo Ogunsina University of Manitoba, Winnipeg R3T 2N2

Keywords:

Massularia acuminata, antioxidant, antimicrobial, DPPH, agar dilution, thiophenolic glycoside

Abstract

Background: Massularia acuminata is a small tree or shrub of tropical rainforest. The leaves are used in Nigerian ethno-medicine for the treatment of microbial infections and pharmacological report suggested the leaf extract as possessing antioxidant activity. This study was therefore carried out to determine the most antioxidant and antimicrobial active fraction(s) of Massularia acuminata leaf and the constituent(s) responsible for the activities. Matherials and Methods: The leaf of Massularia acuminata was investigated for in vitro antioxidant and antimicrobial activities, using a 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and agar dilution method respectively. Results: The ethyl acetate fraction demonstrated the best activities among the partitioned fractions tested. Bioassay guided purification of the most active ethyl acetate fraction led to isolation of a new thiophenolic glycoside, characterized as 4-(3´,3´-dihydroxy-1-mercaptopropyl)phenyl glycosylpyranoside. Conclusion: The isolated compound from the leaf of Massularia acuminata demonstrated antioxidant and antimicrobial activities and may be responsible for the activities of leaf extract and its ethyl acetate fraction, hence this may justify its ethnomedicinal use.

Author Biographies

Ayodeji Oluwabunmi Oriola, Obafemi Awolowo University, Ile-Ife, Nigeria

Department of Pharmacognosy

Joseph Adetunji Aladesanmi, Obafemi Awolowo University, Ile-Ife, Nigeria

Department of Pharmacognosy Professor of Pharmacognosy and Natural Products Chemistry

T O Idowu, Obafemi Awolowo University, Ile-Ife, Nigeria

Department of Pharmaceutics Doctor of Pharmaceutical Microbiology

E O Akinkunmi, Obafemi Awolowo University, Ile-Ife, Nigeria

Department of Pharmaceutical Chemistry Doctor of Pharmaceutical Chemistry

Efere Martins Obuotor, Obafemi Awolowo University, Ile-Ife, Nigeria

Department of Biochemistry Doctor of Biochemistry (Toxicology)

Makanjuola Ojo Ogunsina, University of Manitoba, Winnipeg R3T 2N2

Department of Chemistry Teaching Assistant

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Published

2014-01-21

How to Cite

Oriola, A. O., Aladesanmi, J. A., Idowu, T. O., Akinkunmi, E. O., Obuotor, E. M., & Ogunsina, M. O. (2014). A NEW BIOACTIVE THIOPHENOLIC GLYCOSIDE FROM THE LEAF OF MASSULARIA ACUMINATA (G. DON BULLOCK) EX HOYLE (RUBIACEAE). African Journal of Traditional, Complementary and Alternative Medicines, 11(2), 319–323. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/2143

Issue

Vol. 11 No. 2 (2014)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

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