IN VIVO AND IN VITRO EFFECTS OF BIDENS PILOSA L. (ASTERACEAE) LEAF AQUEOUS AND ETHANOL EXTRACTS ON PRIMED-OESTROGENIZED RAT UTERINE MUSCLE.

Authors

  • Longo Frida ENS
  • Rakotonirina Silvère University of Yaoundé I faculty of Sciences
  • Rakotonirina Alice ENS
  • Savineau Jean Pièrre UFR Sciences de la vie University of Bordeaux2

Keywords:

Bidens pilosa L. Leaf, Uterotrophic, Uterotonic, Mouse, Rat.

Abstract

Bidens pilosa L. is an Asteraceae growing in tropical zones, and traditionally utilized worldwide in herbal medicine. The present work is based on its traditional use during child birth as a labour facilitator. In vivo tests of acute toxicity showed a weak toxic effect for both extracts but the toxicity of the ethanol extract (LD50=6.15g/kg) was upper than that of the aqueous extract (LD50=12.30g/kg). The three-days uterotrophic assay on immature mice showed body weight gain followed by a concentration-dependent decrease up to 4mg/g and a concentration dependent uterine wet weight increase. The ethanol extract exhibited the higher body weight gain representing 22.8±0.7%, (P0.001), at the concentration of 500µg/g/day, while the aqueous extract was significantly more efficient on the uterine wet weight gain of 0.24±0.001% (P0.05), at the concentration of 1000µg/g/day. In vitro isometric contraction measurement of oestrogen-primed rat uterine strips showed a significant high aqueous extract-induced contractile effect from 0.03-1.97mg/ml: on the amplitude of contraction (EC50 = 0.44±0.10mg/ml, P0.05), and on the rate (1.21±0.25mg/ml, P0.05). Inspite of the higher effect of the aqueous extract on the tonus (57.23±23%), the ethanol extract showed a high effect (EC50= 0.34±0.09mg/ml, P0.05). The weak toxicity, the estrogenic-like and the oxytocic-like activities observed could explain the empirical use of Bidens pilosa leaf aqueous extract as an uterotonic preparation to enhance labour, probably due to the presence of biologically active compound(s) which act directly on the uterine muscle.

Author Biographies

Longo Frida, ENS

Teacher in the Department of Biological Sciences of Ecole Normale Supérieure of the University of Yaoundé I since 2000

Rakotonirina Silvère, University of Yaoundé I faculty of Sciences

Senior lecturer in the department of animal Biology and physiology of the faculty of sciences, University of Yaoundé I

Rakotonirina Alice, ENS

Senior lecturer in the department of biological sciences ENS, University of Yaoundé I

Savineau Jean Pièrre, UFR Sciences de la vie University of Bordeaux2

Full Professor at the UFR of Life Sciences University of Bordeaux2

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Published

2007-09-04

How to Cite

Frida, L., Silvère, R., Alice, R., & Jean Pièrre, S. (2007). IN VIVO AND IN VITRO EFFECTS OF BIDENS PILOSA L. (ASTERACEAE) LEAF AQUEOUS AND ETHANOL EXTRACTS ON PRIMED-OESTROGENIZED RAT UTERINE MUSCLE. African Journal of Traditional, Complementary and Alternative Medicines, 5(1), 79–91. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/239

Issue

Section

Research Papers