COMPARATIVE PROFILING OF BIOMARKER PSORALEN IN ANTIOXIDANT ACTIVE EXTRACTS OF DIFFERENT SPECIES OF GENUS FICUS BY VALIDATED HPTLC METHOD

Authors

  • Perwez Alam Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia
  • Omer A. Basudan Deptt. of Pharmacognosy, College of Pharmacy, King Saud University
  • Saleh I. Alqasoumi Deptt. of Pharmacognosy, College of Pharmacy, King Saud University
  • Maged S. Abdel-Kader Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, KSA; Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria, Egypt.
  • Abd El Raheim M. Donia Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, KSA.; Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt.
  • Adnan Al-Rehaily Deptt. of Pharmacognosy, College of Pharmacy, King Saud University
  • Prawez Alam Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University
  • Nasir A. Siddiqui Deptt. of Pharmacognosy, College of Pharmacy, King Saud University
  • Faiyaz Shakeel

DOI:

https://doi.org/10.21010/ajtcam.v12i1.9

Keywords:

Ficus species, psoralen, antioxidant, HPTLC, Validation.

Abstract

Background: A simple but sensitive HPTLC method was developed for the comparative evaluation of psoralen in antioxidant active extracts of leaves of five different species of genus Ficus (Ficus carica, Ficus nitida, Ficus ingens, Ficus palmata and Ficus vasta). Materials and Methods: HPTLC studies were carried out using CAMAG HPTLC system on Glass-backed silica gel 60F254 HPTLC pre-coated plates using selected mobile phase toluene: methanol (9:1). The antioxidant activity was carried out, using DPPH free radical method. Results: Among all the five species of genus Ficus, F. palmata and F. carica exhibited comparatively good antioxidant activity in DPPH assay. The developed HPTLC method was found to give a compact spot for psoralen (Rf = 0.55±0.001) at 305 nm. The regression equation and r2 for psoralen was found to be Y= 4.516X+35.894 and 0.998. The quantification result revealed the presence of psoralen in only two species, F. carica (0.24%, w/w) and F. palmata (1.88%, w/w) which supported their supremacy for anti-oxidant potential over other species. The statistical analysis proved that the developed method was reproducible and selective. Conclusion: The developed method can be used as an important tool to assure the therapeutic dose of active ingredients in herbal formulations as well as for standardization and quality control of bulk drugs and in-process formulations. This method can also be employed for the further study of degradation kinetics and determination of psoralen in plasma and other biological fluids.

Author Biographies

Perwez Alam, Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

Department of Pharmacognosy, Assistant Professor

Omer A. Basudan, Deptt. of Pharmacognosy, College of Pharmacy, King Saud University

Deptt. of Pharmacognosy and Assistant Professor

Saleh I. Alqasoumi, Deptt. of Pharmacognosy, College of Pharmacy, King Saud University

Deptt. of Pharmacognosy and Associate Professor

Maged S. Abdel-Kader, Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, KSA; Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria, Egypt.

Department of Pharmacognosy; Professor

Abd El Raheim M. Donia, Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, KSA.; Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt.

Department of Pharmacognosy, Assistant Professor

Adnan Al-Rehaily, Deptt. of Pharmacognosy, College of Pharmacy, King Saud University

Department of Pharmacognosy; Professor

Prawez Alam, Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University

Department of Pharmacognosy, Assistant Professor

Nasir A. Siddiqui, Deptt. of Pharmacognosy, College of Pharmacy, King Saud University

Deptt. of Pharmacognosy, Associate Professor

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Published

2014-11-29

How to Cite

Alam, P., Basudan, O. A., Alqasoumi, S. I., Abdel-Kader, M. S., Donia, A. E. R. M., Al-Rehaily, A., Alam, P., Siddiqui, N. A., & Shakeel, F. (2014). COMPARATIVE PROFILING OF BIOMARKER PSORALEN IN ANTIOXIDANT ACTIVE EXTRACTS OF DIFFERENT SPECIES OF GENUS FICUS BY VALIDATED HPTLC METHOD. African Journal of Traditional, Complementary and Alternative Medicines, 12(1), 57–67. https://doi.org/10.21010/ajtcam.v12i1.9

Issue

Vol. 12 No. 1 (2015)

Section

Research Papers

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