PROTECTIVE EFFECT OF MEXICAN PROPOLIS ON MDBK CELLS EXPOSED TO AUJESZKY'S DISEASE VIRUS (PSEUDORABIES VIRUS).

Abstract

In this work, antiviral effect of Mexican propolis was evaluated in vitro on MDBK (Madin Darby Bovine Kidney) cell cultures infected with pseudorabies virus (PRV). First was carried the cytotoxicity test of the ethanol extract of propolis (EEP) on this cell culture, subsequently, the infective dose of virus Shope strain was determined for the infection of cell cultures and finally the interaction between EPP and virus was done at cell culture level (two hours before, during and two hours later of infection). Also, cell culture samples with the same treatments were processed for transmission electron microscopy (TEM) to observe possible alterations of propolis on the virus. It was determined that using the treatment of 0.5 mg / ml of EEP two hours before being infected, the culture shows a reduction in the number of plaque forming units compared to the other treatments or with the culture infected, it is important to note that the difference found was statistically significant (p <0.01). Moreover, structurally modified viral particles were observed by TEM, suggesting a possible damage to proteins of viral envelope causing a destabilization of the viral structure which could affect the penetration and replicative cycle of virus. This result opens research perspectives in order to explore the possibility of using propolis as a preventive method against pseudorabies disease.