EVIDENCE-GUIDED OPTIMIZATION OF HERBAL FORMULA CHEONG-HWA-BO-UM-TANG USING MULTIPLEX CYTOKINE PROFILING

Authors

  • Ji Young Lee College of Korean Medicine, Gachon Univeristy
  • Wansu Park College of Korean Medicine, Gachon Univeristy

DOI:

https://doi.org/10.21010/ajtcam.v13i2.9

Keywords:

Cheong-Hwa-Bo-Um-Tang, Lipopolysaccharide, Inflammation, Macrophages, Cytokine, Nitric Oxide

Abstract

Background: Cheong-Hwa-Bo-Um-Tang (CHB) has been traditionally used to treat pharyngolaryngitis and throat inflammation in Korea. However, the anti-inflammatory activity of CHB and the exact component proportion ratio of CHB have not been fully reported. Materials and Methods: This study was conducted to optimize the component proportion ratio of CHB on the basis of experimental evidence using multiplex cytokine profiling assay in LPS-stimulated RAW 264.7 mouse macrophages. Results: The optimized CHB (named Chunhwabun-Cheong-Hwa-Bo-Um-Tang) inhibited the production of NO, IL-6, MCP-1, GMCSF, IL-10, IP-10, VEGF, M-CSF, RANTES, MIP-1β, LIF, LIX, and TNF-α in LPS-stimulated RAW 264.7 mouse macrophages without decreasing cell viability (P<0.05). Chunhwabun-Cheong-Hwa-Bo-Um-Tang consisted of roots of Scrophularia buergeriana, Trichosanthes kirilowii, Anemarrhena asphodeloides, Phellodendron amurense, Angelica gigas, Rehmannia glutinosa, Paeonia japonica, Cnidium officinale, and Glycyrrhiza uralensis (in the ratio of 8: 3: 3: 3: 3: 4: 4: 3: 3). Conclusion: The results of this study indicate that the multiplex cytokine profiling assay could be one of the effective methods for evidence-guided optimization of the component proportion ratio of a traditional herbal formula used for treating excessive inflammation such as the cytokine storm.

Author Biographies

Ji Young Lee, College of Korean Medicine, Gachon Univeristy

Dept. of Pathology (Ph.D.)

Wansu Park, College of Korean Medicine, Gachon Univeristy

Professor of Pathology

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Published

2016-02-05

How to Cite

Lee, J. Y., & Park, W. (2016). EVIDENCE-GUIDED OPTIMIZATION OF HERBAL FORMULA CHEONG-HWA-BO-UM-TANG USING MULTIPLEX CYTOKINE PROFILING. African Journal of Traditional, Complementary and Alternative Medicines, 13(2), 72–80. https://doi.org/10.21010/ajtcam.v13i2.9

Issue

Vol. 13 No. 2 (2016)

Section

Research Papers

License

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