EFFECTS OF POLLEN TYPHAE TOTAL FLAVONE ON β-ARRESTIN-2/SRC/ AKT SIGNALING IN ADIPOSE TISSUES OF TYPE 2 DIABETIC RATS

Authors

  • Xiao-Tao Feng
  • Lin-Na Zhai
  • Chun-Ling Wang
  • Wei Zhao
  • Qun Chen
  • Xiao-Qi Huang Molecular Biology Laboratory, The First Affiliated Hospital, Guangxi University of Chinese Medicine

DOI:

https://doi.org/10.21010/ajtcam.v12i5.11

Keywords:

Chinese medicine, type 2 diabetes, signal transduction, insulin resistance, β-arrestin-2

Abstract

Background: Pollen Typhae total flavone (PTF), the extract from Pollen Typhae, a Chinese herbal medicine, has been reported to improve insulin resistance in type 2 diabetic rats, but the potential mechanisms keep unclear. Materials and Methods: Type 2 diabetic model rats were induced by high-fat diet and low-dose streptozotocin, and then were administered PTF by intragastrical gavage. After treatment for 4 weeks, insulin receptor-β level in adipose tissues was determined by ELISA, and the protein expression was analyzed by western blotting. Results: Administration of PTF increased insulin receptor-β level and enhanced β-arrestin-2 protein expression in adipose tissues of type 2 diabetic rats. Although having no effect on the protein expression of Src or Akt, PTF promoted phosphorylation of Src at Tyr416 and Akt at Ser473. Conclusion: The results indicate that PTF has beneficial effects on the β-arrestin-2/Src/Akt signaling in adipose tissues of type 2 diabetic rats, implying the underlying mechanisms of PTF in ameliorating insulin resistance.

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Published

2015-07-31

How to Cite

Feng, X.-T., Zhai, L.-N., Wang, C.-L., Zhao, W., Chen, Q., & Huang, X.-Q. (2015). EFFECTS OF POLLEN TYPHAE TOTAL FLAVONE ON β-ARRESTIN-2/SRC/ AKT SIGNALING IN ADIPOSE TISSUES OF TYPE 2 DIABETIC RATS. African Journal of Traditional, Complementary and Alternative Medicines, 12(5), 74–78. https://doi.org/10.21010/ajtcam.v12i5.11

Issue

Vol. 12 No. 5 (2015)

Section

Short communications

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