INFLUENCE OF MITRAGYNA CILIATA (MYTA) ON THE MICROSOMAL ACTIVITY of ATPase Na+/K+ DEPENDENT EXTRACT ON A RABBIT HEART

Authors

  • Alain dit Philippe Bidie
  • Ernest Koffi
  • Jean-David N’Guessan
  • Joseph Allico Djaman
  • Frédérique Guede-Guina

Keywords:

Mitragyna ciliata, ATPase Na /K , inhibitors of ATPase Na /K .

Abstract

Mitragyna ciliata (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity of the digitalic type on rat’s isolated heart. In this work we studied the effect of MYTA on microsomal Na+/K+ -dependant ATPase (Na+, K+ ATPase) extracted from the heart of a rabbit since digitalics inhibit Na+, K+ ATPase. Our results revealed that the Na+/K+ ATPase has an optimum pH of 7.4 and temperature of 37oC respectively. There is a linear relationship between the organic phosphate formed and the incubation time over 25 mins incubation period. The ATP hydrolysis rate in the presence of MYTA was 0.775 µM/min. LINEWEAVER and BURK plots showed that MYTA did not alter KM (1.31 mM) but decreased VMAX. This study shows that MYTA exerts a non-competitive inhibition on the microsomal Na+/K+ ATPase extracted from rabbit heart with a Ci50 of 48 µg / ml. We conclude that the mechanism of action of MYTA is linked to the inhibition of the Na+/K+ ATPase like cardiotonics of the digitalic type.

Author Biographies

Alain dit Philippe Bidie

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

Ernest Koffi

Laboratoire de Biochimie et de Sciences des Aliments ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire.

Jean-David N’Guessan

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

Joseph Allico Djaman

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

Frédérique Guede-Guina

Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d’Ivoire

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Published

2008-04-08

How to Cite

Bidie, A. dit P., Koffi, E., N’Guessan, J.-D., Djaman, J. A., & Guede-Guina, F. (2008). INFLUENCE OF MITRAGYNA CILIATA (MYTA) ON THE MICROSOMAL ACTIVITY of ATPase Na+/K+ DEPENDENT EXTRACT ON A RABBIT HEART. African Journal of Traditional, Complementary and Alternative Medicines, 5(3), 294–301. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/423

Issue

Vol. 5 No. 3 (2008)

Section

Research Papers

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