ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF OCIMUM BASILICUM L. AND OBSERVATION OF THE INHIBITION EFFECT ON BACTERIAL CELLS BY USE OF SCANNING ELECTRON MICROSCOPY

Authors

  • Ilhan Kaya
  • Nazife YiÄŸit
  • Mehlika Benli

Keywords:

Antimicrobial activity, Disc diffusion method, Ocimum basilicum, Plant extract, SEM, Spices.

Abstract

The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of Ocimum basilicum L. were studied. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. The results indicated that the methanol extracts of O. basilucum exhibited the antimicrobial activity against tested microorganisms. While the chloroform and acetone extracts had no effect, the methanol extracts showed inhibition zones against strains of Pseudomonas aeruginosa, Shigella sp., Listeria monocytogenes, Staphylococcus aureus and two different strains of Escherichia coli. The cells of microorganisms, which were treated and untreated with plant extracts, were observed by using the scanning electron microscope. It was observed that the treated cells were damaged.

Author Biographies

Ilhan Kaya

Yuzuncuyil University, Faculty of Agriculture, Department of Plant Protection, Van, Turkey

Nazife YiÄŸit

Kirikkale University, Faculty of Science and Arts, of Biology, Kirikkale, Turkey

Mehlika Benli

Ankara University, Faculty of Science, Department of Biology, Ankara, Turkey

Downloads

Published

2008-05-12

How to Cite

Kaya, I., YiÄŸit, N., & Benli, M. (2008). ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF OCIMUM BASILICUM L. AND OBSERVATION OF THE INHIBITION EFFECT ON BACTERIAL CELLS BY USE OF SCANNING ELECTRON MICROSCOPY. African Journal of Traditional, Complementary and Alternative Medicines, 5(4), 363–369. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/440

Issue

Vol. 5 No. 4 (2008)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.