IN VITRO ANTI-INFLAMMATORY ACTIVITY OF THE COMPONENTS OF AMOMUM TSAO-KO IN MURINE MACROPHAGE RAW 264.7 CELLS

Authors

  • Chun Whan Choi Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Gyeonggi-do 16229, Korea
  • Ju Young Shin Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Gyeonggi-do 16229, Korea
  • Changon Seo Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Gyeonggi-do 16229, Korea
  • Seong Su Hong Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Gyeonggi-do 16229, Korea
  • Eun-Kyung Ahn Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Gyeonggi-do 16229, Korea
  • Young Hoon Jung Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Gyeonggi-do 16229, Korea
  • Joa Sub Oh College of Pharmacy, Dankook University, Chungnam 31116, Korea.

DOI:

https://doi.org/10.21010/ajtcamv15i2.4

Keywords:

Amomum tsao-ko, Anti-inflammatory activity, Phytochemical, Cytokines, NF-κB

Abstract

Background: Plants still remain the prime source of drugs for the treatment of inflammation and can provide leads for the development of novel anti-inflammatory agents. Material and methods: An in vitro bioassay guide revealed that the 80% ethanol (EtOH) extract of the whole plant, Amomum tsao-ko (Zingiberaceae), displayed anti-inflammatory activity after assessing its effects on murine macrophage RAW 264.7 cells. Result: Phytochemical study of the 80% EtOH extract of Amomum tsao-ko led to the isolation of eight compounds: 4-hydroxy-3-methoxy-benzoic acid (1), meso-hannokinol (2), (+)-hannokinol (3), coumaric acid (4), 4-hydroxy-benzoic acid (5), (+)-epicatechin (6), (-)-catechin (7), and myrciaphenone A (8). The results indicated that two of the isolated components, (+)-epicatechin (6) and (-)-catechin (7), inhibited the production of nitric oxide (NO) significantly in lipopolysaccharide treated RAW 264.7 cells. Conclusion: LPS-induced interleukin tumor necrosis factor-alpha (TNF-), IL-1β and IL-10 production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that (+)-epicatechin (6) and (-)-catechin (7) reduced the expression of inducible nitric oxide synthase and inhibited nuclear localization of nuclear factor kappa-B (NF-κB).

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Published

2018-02-23

How to Cite

Choi, C. W., Shin, J. Y., Seo, C., Hong, S. S., Ahn, E.-K., Jung, Y. H., & Oh, J. S. (2018). IN VITRO ANTI-INFLAMMATORY ACTIVITY OF THE COMPONENTS OF AMOMUM TSAO-KO IN MURINE MACROPHAGE RAW 264.7 CELLS. African Journal of Traditional, Complementary and Alternative Medicines, 15(2), 26–34. https://doi.org/10.21010/ajtcamv15i2.4

Issue

Vol. 15 No. 2 (2018)

Section

Research Papers

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