DUKKU AND BAEKAM SPRINGWATER INHIBIT THE UREASE ACTIVITY OF HELICOBACTER PYLORI

Authors

  • SungSook Choi College of Pharmacy, Sahmyook University, Seoul, Korea
  • HaeKyung Lee Department of Laboratory Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea
  • JinWoo Kim Department of Dermatology, Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea
  • JiHan Yu Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
  • HyunHo Choi Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
  • HyungKeun Kim Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
  • SangWoo Kim Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
  • Hiun Suk Chae Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

DOI:

https://doi.org/10.21010/ajtcam.v16i1.4

Keywords:

Springwater, H. pylori, Urease activity, Western blot.

Abstract

Background: Springwater (SW) contains many kinds of minerals such as sodium, potassium and copper. These metallic ions may influence the activity of metallo-enzymes such as urease via competitive inhibition. In this study, we investigated the effect of SW on the inhibition of Ni-containing urease activity, which is essential for the colonization of Helicobacter pylori (H. pylori) in the human stomach.
Materials and Methods: We studied the growth inhibition of H. pylori by SW. We evaluated ammonia production to detect urease activity and performed western blot analysis of UreA and UreB for enzyme production.
Results: SW had no significant effect on bacterial growth. Western blot analysis also showed that SW did not affect the translation of UreA and UreB, but it significantly reduced the urease activities of the Jack bean as well as that of H.pylori from 50 to 75%.
Conclusion: These results might indicate that the consumption of SW may prevent the colonization of H. pylori andameliorate the toxic effect on gastric mucosa via the inhibition of urease activity.

Author Biographies

SungSook Choi, College of Pharmacy, Sahmyook University, Seoul, Korea

College of Pharmacy, Sahmyook University, Seoul, Korea

HaeKyung Lee, Department of Laboratory Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

Department of Laboratory Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

JinWoo Kim, Department of Dermatology, Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

Department of Dermatology, Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea

JiHan Yu, Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

HyunHo Choi, Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic
University of Korea, Seoul, Korea.

HyungKeun Kim, Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

SangWoo Kim, Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Hiun Suk Chae, Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Department of Internal Medicine Uijongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

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Published

2019-02-12

How to Cite

Choi, S., Lee, H., Kim, J., Yu, J., Choi, H., Kim, H., Kim, S., & Chae, H. S. (2019). DUKKU AND BAEKAM SPRINGWATER INHIBIT THE UREASE ACTIVITY OF HELICOBACTER PYLORI. African Journal of Traditional, Complementary and Alternative Medicines, 16(1), 34–40. https://doi.org/10.21010/ajtcam.v16i1.4

Issue

Vol. 16 No. 1 (2019)

Section

Research Papers

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