ISOLATION AND CHARACTERIZATION OF ANTINEOPLASTIC ALKALOIDS FROM CATHARANTHUS ROSEUS L. DON. CULTIVATED IN EGYPT

Authors

  • K. A. Shams
  • N. M. Nazif
  • N. S. Abdel Azim
  • K. A. Abdel Shafeek
  • M. L. El-Missiry
  • S. I. Ismail
  • M. M. Seif El Nasr

Keywords:

Catharanthus roseus L., Apocyanaceae, Vinblastine, Vincrisitine, Antileuckemic alkaloids, VLC, HPLC, HPTLC.

Abstract

Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).

Author Biography

K. A. Shams

Phytochemistry Department, National Research Centre, El bohooth St., Dokki, Giza, Egypt, 12311.

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Published

2009-03-07

How to Cite

Shams, K. A., Nazif, N. M., Abdel Azim, N. S., Abdel Shafeek, K. A., El-Missiry, M. L., Ismail, S. I., & Seif El Nasr, M. M. (2009). ISOLATION AND CHARACTERIZATION OF ANTINEOPLASTIC ALKALOIDS FROM CATHARANTHUS ROSEUS L. DON. CULTIVATED IN EGYPT. African Journal of Traditional, Complementary and Alternative Medicines, 6(2), 118–122. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/568

Issue

Vol. 6 No. 2 (2009)

Section

Research Papers

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