ANTIFUNGAL ACTIVITY OF CRUDE EXTRACTS OF GLADIOLUS DALENII VAN GEEL (IRIDACEAE)

Authors

  • Judith A. Odhiambo University of Nairobi
  • George M. Siboe University of Nairobi
  • Catherine W. Lukhoba University of Nairobi
  • Saffudin F. Dossaji University of Nairobi

Keywords:

Gladiolus dalenii, Aspergillus niger, Antifungal activity

Abstract

Bulb extracts of Gladiolus dalenii reportedly used in the treatment of fungal infections in HIV/AIDS patients in the Lake Victoria region were tested for antifungal activity using the disc diffusion assay technique. Commercially used antifungal drugs, Ketaconazole and Griseofulvin (Cosmos Pharmaceuticals) were used as standards. Dichloromethane (CH2CL2)/Methanol (MeOH) in the ratio 1:1. Soluble extracts showed antifungal activity against Aspergillus niger. Direct bioautography on silica gel Thin Layer Chromatography (TLC) and appropriate spraying agents were used to identify the active component in the extract. The activities of both the extracts were higher than that of Griseofulvin. CH2CL2 soluble extract in addition showed ability to delay sporulation in A.niger. The active group of compounds in the extracts was identified as alkaloids, which offer immense potential for development of new and valuable pharmaceutical products.

Author Biographies

Judith A. Odhiambo, University of Nairobi

Postgraduate/Teaching assistant/Demonstrator School of Biological Sciences

George M. Siboe, University of Nairobi

Professor School of Biological Sciences

Catherine W. Lukhoba, University of Nairobi

Lecturer School of Biological Sciences

Saffudin F. Dossaji, University of Nairobi

Senior Lecturer School of Biological Sciences

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Published

2009-08-22

How to Cite

Odhiambo, J. A., Siboe, G. M., Lukhoba, C. W., & Dossaji, S. F. (2009). ANTIFUNGAL ACTIVITY OF CRUDE EXTRACTS OF GLADIOLUS DALENII VAN GEEL (IRIDACEAE). African Journal of Traditional, Complementary and Alternative Medicines, 7(1), 53–58. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/965

Issue

Vol. 7 No. 1 (2010)

Section

Research Papers

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