DETECTION OF ANTIMICROBIAL COMPOUNDS BY BIOAUTOGRAPHY OF DIFFERENT EXTRACTS OF LEAVES OF SELECTED SOUTH AFRICAN TREE SPECIES

Authors

  • M. M. Suleiman
  • L. J. McGaw
  • J. N. Eloff

Keywords:

Bioautography, Medicinal plants, Antifungal, Antibacterial, Synergism

Abstract

The hexane, acetone, dichloromethane and methanol extracts of Combretum vendae A.E. van Wyk (Combretaceae), Commiphora harveyi (Engl.) Engl. (Burseraceae), Khaya anthotheca (Welm.) C.DC (Meliaceae), Kirkia wilmsii Engl. (Kirkiaceae), Loxostylis alata A. Spreng. ex Rchb. (Anacardiaceae), Ochna natalitia (Meisn.) Walp. (Ochnaceae) and Protorhus longifolia (Bernh. Ex C. Krauss) Engl. (Anacardiaceae) were screened for their antimicrobial activity. The test organisms included bacteria (Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus), and fungi (Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Microsporum canis and Sporothrix schenckii). A simple bioautographic procedure, involving spraying suspensions of the bacteria or fungi on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial and antifungal compounds present in the extracts. All the extracts had antimicrobial activity against at least one of the test microorganisms. This activity was denoted by white spots against a red-purple background on the TLC plates after spraying with tetrazolium violet. Twenty seven TLC plates; 9 for each solvent system and 3 different solvent systems per organism were tested in the bioautographic procedure. Of the bacteria tested, S. aureus was inhibited by the most compounds separated on the TLC plates from all the tested plants. Similarly, growth of the fungus C. neoformans was also inhibited by many compounds present in the extracts. Loxostylis alata appeared to be the plant extract with the highest number of inhibition bands when compared with other plants tested against both bacteria and fungi. This species was selected for in depth further study.

Author Biography

J. N. Eloff

Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa

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Published

2009-08-22

How to Cite

Suleiman, M. M., McGaw, L. J., & Eloff, J. N. (2009). DETECTION OF ANTIMICROBIAL COMPOUNDS BY BIOAUTOGRAPHY OF DIFFERENT EXTRACTS OF LEAVES OF SELECTED SOUTH AFRICAN TREE SPECIES. African Journal of Traditional, Complementary and Alternative Medicines, 7(1), 64–78. Retrieved from https://athmsi.org/journals/index.php/ajtcam/article/view/998

Issue

Vol. 7 No. 1 (2010)

Section

Research Papers

License

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