Vol 8 No 1 (2014)
Articles

ASPERGILLUS MONITORING PROJECT IN A LARGE EDUCATIONAL HOSPITAL USING MOLECULAR

Kambiz - DIBA
Urmia University of Medical Sciences
Bio
Mohamad H Rahimirad
Urmia University of Medical Sciences
Bio
khadijeh H Makhdoomi
Urmia University of Medical Sciences
Bio
N F Eslamloo
Golestan University of Medical Sciences
Bio
Published October 6, 2013
Keywords
  • Aspergillus,
  • identification,
  • molecular,
  • hospital
How to Cite
DIBA, K., Rahimirad, M., Makhdoomi, khadijeh, & Eslamloo, N. (2013). ASPERGILLUS MONITORING PROJECT IN A LARGE EDUCATIONAL HOSPITAL USING MOLECULAR. African Journal of Infectious Diseases (AJID), 8(1), 1-4. Retrieved from https://athmsi.org/journals/index.php/AJID/article/view/1519

Abstract

Our main object was to use a rapid and cheap molecular method for monitoring of Aspergillus infections and epidemiological approaches. Different molecular methods such as restriction fragment length polymorphism based on amplification of ribosomal RNA have been employed to identify Aspergilli in the level of species. The subject of our study was a group of hospitalized patients with clinical and subclinical signs of infection. All of the collected clinical specimens were transported to the medical mycology lab and examined for Aspergillus identification. Environmental specimens were collected from air and surfaces inspected for the Aspergillus hospital sources. A morphological study was firstly performed including; growth characteristics and microscopic features of Aspergillus species on mycological media for the identification. For the confirmation of Aspergillus isolates which similarly found in clinical and environmental sources, molecular method polymerase chain reaction/restriction fragment length polymorphism was carried out. From the mentioned specimens, 110 fungal isolates included Candida spp., Aspergillus spp. and other fungi. Among the clinical isolates of Aspergilli; Aspergillus flavus (47%), Aspergillus fumigatus (29.4%) and Aspergillus niger (23.6%) were the most frequent species respectively, and also environmental Aspergillus isolates were Aspergillus niger (43.7%), Aspergillus flavus (41.8%), Aspergillus fumigatus (14.7%). Because of facility in use, speed and high sensitivity of diagnosis, the polymerase chain reaction/restriction fragment length polymorphism with a single restriction enzyme was very useful in identification of medically important Aspergillus spp.