SEROTYPING AND ANTIMICROBIAL DRUG RESISTANCE OF SALMONELLA ISOLATED FROM LETTUCE AND HUMAN DIARRHEA SAMPLES IN BURKINA FASO

Authors

  • Somda Namwin Siourimè
  • Bonkoungou Ouindgueta Juste Isidore
  • Traoré Oumar
  • Bassolé Ismael Henri Nestor
  • Traoré Yves
  • Barro Nicolas
  • Savadogo Aly

DOI:

https://doi.org/10.21010/ajid.v11i2.4

Keywords:

Salmonella serotypes, antimicrobial susceptibility, lettuce, human

Abstract

Background: In Burkina Faso dirty water in particular those of the stoppings and the gutter ones are used for vegetables irrigation in the gardens. The aim of this study was to determine the prevalence and antibiotic susceptibility of Salmonella serotypes from humans and lettuce samples in Burkina Faso. Materials and Methods: Salmonella strains isolated from patients in 2009 to 2015 and lettuce samples in 2014 in Burkina Faso were serotyped using specific antisera. All strains were subjected to a set of 14 antibiotics to study their antibiogram by using Baeur–Kirby disk diffusion method. Results: Out of 154 Salmonella isolated, 60 were from human and 94 from lettuce samples. Serotyping revealed four different serotypes and 39% (60) untypeable strains from human and lettuce (14 and 46 strains). Salmonella serotypes from human and lettuce samples were: Paratyphi A (10% and 22%), Paratyphi B (34% and 8%), Paratyphi C (14% and 18%) and Typhi (21% and 1%). A high resistance of Salmonella Paratyphi B and Salmonella spp to tetracycline were 70% from human and 35 % from lettuce samples. Multiresistance was observed to tetracycline, chloramphenicol and amoxicillin/clavulanic-acid or ampicillin with Salmonella Paratyphi B 35% and Salmonella Typhi 33% from human samples and Salmonella spp 4% from lettuce samples. Conclusion: This study showed the diversity of Salmonella serotypes from both clinical and environmental samples and emergence of multiresistant Salmonella to antibiotics in Burkina Faso. A lettuce is a potential source of transmission of Salmonella causing diarrhea among human in Burkina Faso.

Author Biography

Somda Namwin Siourimè

Laboratoire de Biochimie et d’Immunologie Appliquée (LABIA). Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN). Département de Biochimie-Microbiologie. UFR en Sciences de la vie et de la terre. Ecole Doctorale Sciences et Technologies. Université Ouaga I Pr Joseph Ki- Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso

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Published

2017-06-08

How to Cite

Siourimè, S. N., Juste Isidore, B. O., Oumar, T., Nestor, B. I. H., Yves, T., Nicolas, B., & Aly, S. (2017). SEROTYPING AND ANTIMICROBIAL DRUG RESISTANCE OF SALMONELLA ISOLATED FROM LETTUCE AND HUMAN DIARRHEA SAMPLES IN BURKINA FASO. African Journal of Infectious Diseases (AJID), 11(2), 24–30. https://doi.org/10.21010/ajid.v11i2.4

Issue

Vol. 11 No. 2 (2017)

Section

Articles

License

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