Vol 15 No 1 (2021)
Articles

DETECTION OF DIARRHEAGENIC ESCHERICHIA COLI IN HUMAN DIARRHEIC STOOL AND DRINKING WATER SAMPLES IN OUAGADOUGOU, BURKINA FASO.

Isidore Juste Ouindgueta Bonkoungo
University Joseph KI-ZERBO, Ouagadougou
Namwin Siourimè Somda
Département Technologie Alimentaire (DTA) / IRSAT / CNRST, Burkina Faso, 03 BP 7047 Ouagadougou 03. Tel : (00226) 25 36 37 90
Oumar Traore
Unité de Formation et de Recherche en Sciences Appliquées à la Technologie (UFR/SAT). Université de Dédougou, BP 176 Dédougou Tel : (00226) 20 52 12 70
Barthelemy Sibiri ZOMA
Polygon Bio Services SARL,09 BP 969 Ouagadougou 09, Ouagadougou, Burkina Faso 969. Tel: (00226) 70 80 82 08
Zakaria Garba
Unité de Recherche Clinique de NANORO, IRSS-CNRST, BP: 218 Ouaga 11 Burkina Faso, Tel : (00226) 25 33 08 81
Maxime Koiné DRABO
Institut de Recherche en Sciences de la santé, CNRST, 03 B.P. 7192 Ouagadougou 03 Burkina Faso, Tel: (00226) 25 36 32 15
Nicolas Barro
Département de Biochimie-Microbiologie. UFR- Sciences de la vie et de la terre. Université Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Published December 14, 2020
Keywords
  • 16-plex PCR; drinking water; diarrhoeagenic Escherichia coli; Burkina Faso.
How to Cite
Ouindgueta Bonkoungo, I., Somda, N., Traore, O., ZOMA, B., Garba, Z., DRABO, M., & Barro, N. (2020). DETECTION OF DIARRHEAGENIC ESCHERICHIA COLI IN HUMAN DIARRHEIC STOOL AND DRINKING WATER SAMPLES IN OUAGADOUGOU, BURKINA FASO. African Journal of Infectious Diseases (AJID), 15(1), 53-58. https://doi.org/10.21010/ajid v15n1.7

Abstract

Background: The presence of diarrheagenic Escherichia coli (DEC) in drinking water, is a grave public health problem. This study was aimed at characterization of diarrheagenic Escherichia coli isolated from drinking water and faecal samples from diarrheic patients in Ouagadougou, Burkina Faso.

Materials and Methods: A total of 242 water samples consisting of 182 potable sachets and 60 from boreholes were collected in the period between October 2018 and April 2019 in the city of Ouagadougou. Faecal samples were also collected from 201 diarrheic patients visiting National Public Health Laboratory for a biological diagnosis by coproculture. The presence of virulence genes associated with DEC was determined by 16-plex polymerase chain reaction from bacteria culture.

Results: From drinking water, we found 17% (42/242) Escherichia coli isolates in which 1% (2/242) DEC were detected. Among analyzed samples (182 sachet water versus 60 borehole water), the two DEC (01 ETEC and 01 EPEC) were detected in sachet water. DEC were detected in 20% (40/201) of patients. Enteroaggregative Escherichia coli (EAEC) were mostly detected in 10% followed by Enteropathogenic Escherichia coli (EPEC) in 4%, Enteroinvasive Escherichia coli (EIEC) in 2%, and Shiga toxin-producing Escherichia coli (STEC) 0.5%. However, Enterotoxigenic Escherichia coli (ETEC) was not detected alone, but in co-infections with EAEC.

Conclusion: The present study documented the prevalence of Escherichia coli pathovars associated in patients with diarrhea, and shows that drinking water might be a source of DEC transmission in human.