PHENOLIC CONSTITUENTS FROM SARCOPYRAMIS NEPALENSIS AND THEIR Α-GLUCOSIDASE INHIBITORY ACTIVITY

Authors

  • Chao Tan
  • Jiangcheng Zuo The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei, China
  • Xiaofang Yi Yiling maternity and child health care hospital, Yichang 443003, Hubei, China;
  • Peng Wang The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei, China;
  • Chunhua Luo The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei, China;
  • Yong Hu The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei, China;
  • Hong Yi The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei, China;
  • Wei Qiao The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei, China;

DOI:

https://doi.org/10.21010/ajtcam.v12i3.20

Keywords:

Natural Products

Abstract

Background: This study was carried out to fully investigate the phenolic chemical constituents of Sarcopyramis nepalensis and determine their α-glucosidase inhibitory activity. Materials and Methods: S. nepalensis was extracted using the ultrasonic assistant extraction (UAE) method and further fractionated with petroleum ether (PE), chloroform (CHCl3), ethyl acetate (EtOAc) and n-butanol (n-BuOH), respectively. The active fraction was chromatographed on AB-8 macroporous resin column, silica gel column, Sephadex LH-20 column, RP-ODS column and semi-preparative HPLC column. The isolated phenolic constituents were identified by 1H-Nuclear Magnetic Resonance (NMR), 13C-NMR and mass spectral (MS) analyses and detected their α-glucosidase inhibitory activity by micro-plate. Results: Ten phenolic constituents were isolated and identified from the active fraction of S. nepalensis. They were identified as isorhamnetin(1), quercetin(2), isorhamnetin-3-O-β-D-glucopyranoside(3), isoquercetin (4), astragalin(5),isorhamnetin-3-O-(6"-p-coumaroyl)-β-D-glucopyranoside(6),isorhamnetin-3-O-(6"-caffeoyl)-β-D-glucopyranoside(7), isoferulic acid(8) Caffeic acid(9) and ellagic acid(10). All of the phenolic compounds were assayed for their hypoglycemic activity against α-glucosidase in vitro. Compound 4, 6 and 7 showed promising α-glucosidase inhibitory activity with the IC50 values of 0.69 mg/ml, 0.56 mg/ml, 0.45 mg/ml, respectively. Conclusion: Compounds 5-8 were isolated for the first time from S. nepalensis. This is the first report on the characterization of phenolic compounds and possible utilization of S. nepalensis for therapeutic intervention in type 2 diabetes.

Downloads

Published

2015-04-05

How to Cite

Tan, C., Zuo, J., Yi, X., Wang, P., Luo, C., Hu, Y., Yi, H., & Qiao, W. (2015). PHENOLIC CONSTITUENTS FROM SARCOPYRAMIS NEPALENSIS AND THEIR Α-GLUCOSIDASE INHIBITORY ACTIVITY. African Journal of Traditional, Complementary and Alternative Medicines, 12(3), 156–160. https://doi.org/10.21010/ajtcam.v12i3.20

Issue

Vol. 12 No. 3 (2015)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.